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cell lines hek293t atcc crl 11268 gko hek293 kind gift  (ATCC)


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    ATCC cell lines hek293t atcc crl 11268 gko hek293 kind gift
    Cell Lines Hek293t Atcc Crl 11268 Gko Hek293 Kind Gift, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+hek293+cell+line+atcc+crl/293T%2F17/pm41961591-408-199-202
    Average 99 stars, based on 12124 article reviews
    cell lines hek293t atcc crl 11268 gko hek293 kind gift - by Bioz Stars, 2026-09
    99/100 stars

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    shRNA:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    Recombinant:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    Isolation:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    Western Blot:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    RNAscope:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    Multiplex Assay:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    Enzyme-linked Immunosorbent Assay:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

    Sequencing:

    Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026



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    r71007 hek293 cell line atcc  (ATCC)
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    sv40 large t antigen atcc crl 3216 wehi 13var mouse fibrosarcoma tnf sensitive reporter cell line atcc crl 2168 hek atp089 hek293 cells  (ATCC)
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    ATCC sv40 large t antigen atcc crl 3216 wehi 13var mouse fibrosarcoma tnf sensitive reporter cell line atcc crl 2168 hek atp089 hek293 cells
    Sv40 Large T Antigen Atcc Crl 3216 Wehi 13var Mouse Fibrosarcoma Tnf Sensitive Reporter Cell Line Atcc Crl 2168 Hek Atp089 Hek293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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